DICP OpenIR
Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field
Cao, Xupeng; Fu, Wantao; Yu, Xingju; Zhang, Wei; Zhang W(张卫); Zhang W(张卫)
关键词Silica Spicule Silicatein Spiculogenesis Archaeocyte-dominant Cell Populations Primmorph Culture Sponge Hymeniacidon Perlevis (Porifera)
刊名CELL AND TISSUE RESEARCH
2007-09-01
DOI10.1007/s00441-007-0443-1
329期:3页:595-608
收录类别SCI
文章类型Article
部门归属18
项目归属1812
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]DEMOSPONGE SUBERITES-DOMUNCULA ; SILICATEIN FILAMENTS ; EPHYDATIA-MUELLERI ; SILICEOUS SPICULES ; PRIMMORPHS ; SPICULOGENESIS ; PROLIFERATION ; SCLEROCYTES ; LECTIN ; LARVAE
英文摘要To characterize the formation of silica spicules, the dynamics of spiculogenesis of an intertidal marine sponge Hymeniacidon perlevis (Montagu 1818) (Porifera: Demospongiae) were investigated by measuring the gene expression of silicatein (the enzyme responsible for spicule silicification) and the dimensional changes of spicules during the developmental process of individual sponges and in cell cultures of primmorphs of archaeocyte-dominant cell populations. The different developmental stages of spicules were documented by time-lapse microscopy and observed by transmission electron microscopy during a 1-month culture period. During its annual life cycle, H. perlevis has four different developmental stages: dormancy, resuscitation, bloom, and decline. Field-grown individual sponge samples at different stages were collected over 7 months (March to September 2005). The dimensions of the silica spicules from these samples were microscopically measured and statistically analyzed. This analysis and the material properties of the spicules allowed them to be classified into four groups representing the different developmental stages of spiculogenesis. Silicatein expression in the bloom stage was more than 100 times higher than that in the other stages and was correlated with the spicule developmental stage. The trend of spicule formation in field-grown sponges was consistent with the trend in cell culture. A new parameter, the maturation degree (MD) of spicules (defined as the ratio of actual to theoretical silica deposition of mature spicules), was introduced to quantify spicule development. Silica spiculogenesis during H. perlevis development was delineated by comparing MD and silicatein expression.
语种英语
原文出处查看原文
WOS记录号WOS:000249128400016
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98715
专题中国科学院大连化学物理研究所
通讯作者Zhang W(张卫); Zhang W(张卫)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 10089, Peoples R China
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Mol Bioproc & Bioprod Lab, Adelaide, SA 5042, Australia
推荐引用方式
GB/T 7714
Cao, Xupeng,Fu, Wantao,Yu, Xingju,et al. Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field[J]. CELL AND TISSUE RESEARCH,2007,329(3):595-608.
APA Cao, Xupeng,Fu, Wantao,Yu, Xingju,Zhang, Wei,张卫,&张卫.(2007).Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field.CELL AND TISSUE RESEARCH,329(3),595-608.
MLA Cao, Xupeng,et al."Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field".CELL AND TISSUE RESEARCH 329.3(2007):595-608.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cao, Xupeng]的文章
[Fu, Wantao]的文章
[Yu, Xingju]的文章
百度学术
百度学术中相似的文章
[Cao, Xupeng]的文章
[Fu, Wantao]的文章
[Yu, Xingju]的文章
必应学术
必应学术中相似的文章
[Cao, Xupeng]的文章
[Fu, Wantao]的文章
[Yu, Xingju]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。