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Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field
Cao, Xupeng; Fu, Wantao; Yu, Xingju; Zhang, Wei; Zhang W(张卫); Zhang W(张卫)
KeywordSilica Spicule Silicatein Spiculogenesis Archaeocyte-dominant Cell Populations Primmorph Culture Sponge Hymeniacidon Perlevis (Porifera)
Source PublicationCELL AND TISSUE RESEARCH
2007-09-01
DOI10.1007/s00441-007-0443-1
Volume329Issue:3Pages:595-608
Indexed BySCI
SubtypeArticle
Department18
Funding Project1812
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectCell Biology
WOS Research AreaCell Biology
WOS KeywordDEMOSPONGE SUBERITES-DOMUNCULA ; SILICATEIN FILAMENTS ; EPHYDATIA-MUELLERI ; SILICEOUS SPICULES ; PRIMMORPHS ; SPICULOGENESIS ; PROLIFERATION ; SCLEROCYTES ; LECTIN ; LARVAE
AbstractTo characterize the formation of silica spicules, the dynamics of spiculogenesis of an intertidal marine sponge Hymeniacidon perlevis (Montagu 1818) (Porifera: Demospongiae) were investigated by measuring the gene expression of silicatein (the enzyme responsible for spicule silicification) and the dimensional changes of spicules during the developmental process of individual sponges and in cell cultures of primmorphs of archaeocyte-dominant cell populations. The different developmental stages of spicules were documented by time-lapse microscopy and observed by transmission electron microscopy during a 1-month culture period. During its annual life cycle, H. perlevis has four different developmental stages: dormancy, resuscitation, bloom, and decline. Field-grown individual sponge samples at different stages were collected over 7 months (March to September 2005). The dimensions of the silica spicules from these samples were microscopically measured and statistically analyzed. This analysis and the material properties of the spicules allowed them to be classified into four groups representing the different developmental stages of spiculogenesis. Silicatein expression in the bloom stage was more than 100 times higher than that in the other stages and was correlated with the spicule developmental stage. The trend of spicule formation in field-grown sponges was consistent with the trend in cell culture. A new parameter, the maturation degree (MD) of spicules (defined as the ratio of actual to theoretical silica deposition of mature spicules), was introduced to quantify spicule development. Silica spiculogenesis during H. perlevis development was delineated by comparing MD and silicatein expression.
Language英语
URL查看原文
WOS IDWOS:000249128400016
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/98715
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang W(张卫); Zhang W(张卫)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 10089, Peoples R China
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Mol Bioproc & Bioprod Lab, Adelaide, SA 5042, Australia
Recommended Citation
GB/T 7714
Cao, Xupeng,Fu, Wantao,Yu, Xingju,et al. Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field[J]. CELL AND TISSUE RESEARCH,2007,329(3):595-608.
APA Cao, Xupeng,Fu, Wantao,Yu, Xingju,Zhang, Wei,张卫,&张卫.(2007).Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field.CELL AND TISSUE RESEARCH,329(3),595-608.
MLA Cao, Xupeng,et al."Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field".CELL AND TISSUE RESEARCH 329.3(2007):595-608.
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