中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field
作者: Cao, Xupeng;  Fu, Wantao;  Yu, Xingju;  Zhang, Wei
通讯作者: 张卫
关键词: silica spicule ;  silicatein ;  spiculogenesis ;  archaeocyte-dominant cell populations ;  primmorph culture ;  sponge, Hymeniacidon perlevis (Porifera)
刊名: CELL AND TISSUE RESEARCH
发表日期: 2007-09-01
DOI: 10.1007/s00441-007-0443-1
卷: 329, 期:3, 页:595-608
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1812
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
英文摘要: To characterize the formation of silica spicules, the dynamics of spiculogenesis of an intertidal marine sponge Hymeniacidon perlevis (Montagu 1818) (Porifera: Demospongiae) were investigated by measuring the gene expression of silicatein (the enzyme responsible for spicule silicification) and the dimensional changes of spicules during the developmental process of individual sponges and in cell cultures of primmorphs of archaeocyte-dominant cell populations. The different developmental stages of spicules were documented by time-lapse microscopy and observed by transmission electron microscopy during a 1-month culture period. During its annual life cycle, H. perlevis has four different developmental stages: dormancy, resuscitation, bloom, and decline. Field-grown individual sponge samples at different stages were collected over 7 months (March to September 2005). The dimensions of the silica spicules from these samples were microscopically measured and statistically analyzed. This analysis and the material properties of the spicules allowed them to be classified into four groups representing the different developmental stages of spiculogenesis. Silicatein expression in the bloom stage was more than 100 times higher than that in the other stages and was correlated with the spicule developmental stage. The trend of spicule formation in field-grown sponges was consistent with the trend in cell culture. A new parameter, the maturation degree (MD) of spicules (defined as the ratio of actual to theoretical silica deposition of mature spicules), was introduced to quantify spicule development. Silica spiculogenesis during H. perlevis development was delineated by comparing MD and silicatein expression.
关键词[WOS]: DEMOSPONGE SUBERITES-DOMUNCULA ;  SILICATEIN FILAMENTS ;  EPHYDATIA-MUELLERI ;  SILICEOUS SPICULES ;  PRIMMORPHS ;  SPICULOGENESIS ;  PROLIFERATION ;  SCLEROCYTES ;  LECTIN ;  LARVAE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000249128400016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98715
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 10089, Peoples R China
3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Mol Bioproc & Bioprod Lab, Adelaide, SA 5042, Australia

Recommended Citation:
Cao, Xupeng,Fu, Wantao,Yu, Xingju,et al. Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field[J]. CELL AND TISSUE RESEARCH,2007,329(3):595-608.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Cao, Xupeng]'s Articles
 [Fu, Wantao]'s Articles
 [Yu, Xingju]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Cao, Xupeng]‘s Articles
 [Fu, Wantao]‘s Articles
 [Yu, Xingju]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace