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题名: Capillary trap column with strong cation-exchange monolith for automated shotgun proteome analysis
作者: Wang, Fangjun;  Dong, Jing;  Jiang, Xiaogang;  Ye, Mingliang;  Zou, Hanfa
通讯作者: 邹汉法
刊名: ANALYTICAL CHEMISTRY
发表日期: 2007-09-01
DOI: 10.1021/ac070736f
卷: 79, 期:17, 页:6599-6606
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A 150 mu m internal diameter capillary monolithic column with a strong cation-exchange stationary phase was prepared by direct in situ polymerization of ethylene glycol methacrylate phosphate and bisacrylamide in a trinary porogenic solvent consisting dimethylsulfoxide, dodecanol, and N,N '-dimethylformamide. This phosphate monolithic column exhibits higher dynamic binding capacity, faster kinetic adsorption of peptides, and more than 10 times higher permeability than the column packed with commercially available strong cation-exchange particles. It was applied as a trap column in a nanoflow liquid chromatography-tandem mass spectrometry system for automated sample injection and online multidimensional separation. It was observed that the sample could be loaded at a flow rate as high as 40 mu L/min with a back pressure of similar to 1300 psi and without compromising the separation efficiency. Because of its good orthogonality to the reversed phase separation mechanism, the phosphate monolithic trap column was coupled with a reversed-phase column for online multidimensional separation of 19 mu g of the tryptic digest of yeast proteins. A total of 1522 distinct proteins were identified from 5608 unique peptides (total of 54 780 peptides) at the false positive rate only 0.46%.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ORGANIC POLYMER MONOLITHS ;  TANDEM MASS-SPECTROMETRY ;  HUMAN PLASMA PROTEOME ;  STATIONARY PHASES ;  MULTIDIMENSIONAL CHROMATOGRAPHY ;  NANOELECTROSPRAY IONIZATION ;  IDENTIFICATION TECHNOLOGY ;  SURFACE-CHEMISTRY ;  MEMBRANE-PROTEINS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000249242900029
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98731
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Fangjun,Dong, Jing,Jiang, Xiaogang,et al. Capillary trap column with strong cation-exchange monolith for automated shotgun proteome analysis[J]. ANALYTICAL CHEMISTRY,2007,79(17):6599-6606.
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