DICP OpenIR
Eicosapentaenoic acid modifies lipid composition in caveolae and induces translocation of endothelial nitric oxide synthase
Li, Qiurong; Zhang, Qiang; Wang, Meng; Zhao, Sumin; Ma, Jian; Luo, Nan; Li, Ning; Li, Yousheng; Xu, Guowang; Li, Jieshou; Li JS(黎介寿); Li JS(黎介寿)
关键词Eicosapentaenoic Acid Polyunsaturated Fatty Acids Caveolae Fatty Acid Composition Phospholipids Enos
刊名BIOCHIMIE
2007
DOI10.1016/j.biochi.2006.10.009
89期:1页:169-177
收录类别SCI
文章类型Article
部门归属18
项目归属1808
产权排名2;4
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]PROTEIN-KINASE B ; LOCALIZATION ; PHOSPHORYLATION ; ACTIVATION ; RAFTS ; OMEGA-3-FATTY-ACIDS ; ATHEROSCLEROSIS ; MICRODOMAINS ; MEMBRANES ; SYSTEM
英文摘要Endothelial nitric oxide synthase (eNOS) plays a crucial role in the regulation of a variety of cardiovascular functions. Many studies have shown that dietary n-3 polyunsaturated fatty acids (PUFAs) have beneficial effects on coronary atherosclerosis. However, the mechanisms of n-3 PUFAs regulation in eNOS activation remain unknown. In the present study we investigated the effects of eicosapentaenoic acid (EPA, 20:5 n-3) on subcellular distribution of eNOS and lipid composition of caveolae. We demonstrated for the first time that EPA treatment profoundly altered lipid composition and fatty acyl substitutions of phospholipids in caveolae. We found that caveolin-1 was solely located in caveolae fractions in control cells, and EPA treatment displaced caveolin-1 from caveolae. eNOS was detected in the caveolin-enriched fractions and noncaveolae fractions in control cells. EPA treatment induced the translocation of eNOS from caveolae fractions to soluble fractions. P-eNOS was also distributed in both fractions. After EPA treatment, the level of p-eNOS in each fraction was increased but the distribution of which was unaffected. Moreover, the results of immunofluorescence confirmed that EPA could redistribute caveolin-1 and eNOS in plasma membrane. eNOS activity in HUVEC cells was increased after EPA treatment, which was in a dose dependent manner. And incubation with 50 mu M EPA had the maximum effect on eNOS activity. Our results suggested that eNOS translocation was paralleled by a stimulated capacity for NO production in the cells. We found that total Akt and p-Akt were primarily presented in heavy membranes in control cells, and the relative level of p-Akt increased but the distribution did not change after EPA treatment. The distribution of CaM was slightly changed after EPA treatment.
语种英语
原文出处查看原文
WOS记录号WOS:000243630100015
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98777
专题中国科学院大连化学物理研究所
通讯作者Li JS(黎介寿); Li JS(黎介寿)
作者单位1.Jinling Hosp, Inst Gen Surg, Nanjing 210002, Peoples R China
2.Chinese Acad Sci, Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
3.Nanjing Univ, Sch Med, Nanjing 210093, Peoples R China
推荐引用方式
GB/T 7714
Li, Qiurong,Zhang, Qiang,Wang, Meng,et al. Eicosapentaenoic acid modifies lipid composition in caveolae and induces translocation of endothelial nitric oxide synthase[J]. BIOCHIMIE,2007,89(1):169-177.
APA Li, Qiurong.,Zhang, Qiang.,Wang, Meng.,Zhao, Sumin.,Ma, Jian.,...&黎介寿.(2007).Eicosapentaenoic acid modifies lipid composition in caveolae and induces translocation of endothelial nitric oxide synthase.BIOCHIMIE,89(1),169-177.
MLA Li, Qiurong,et al."Eicosapentaenoic acid modifies lipid composition in caveolae and induces translocation of endothelial nitric oxide synthase".BIOCHIMIE 89.1(2007):169-177.
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