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题名: Eicosapentaenoic acid modifies lipid composition in caveolae and induces translocation of endothelial nitric oxide synthase
作者: Li, Qiurong;  Zhang, Qiang;  Wang, Meng;  Zhao, Sumin;  Ma, Jian;  Luo, Nan;  Li, Ning;  Li, Yousheng;  Xu, Guowang;  Li, Jieshou
通讯作者: 黎介寿
关键词: eicosapentaenoic acid ;  polyunsaturated fatty acids ;  caveolae ;  fatty acid composition ;  phospholipids ;  eNOS
刊名: BIOCHIMIE
发表日期: 2007
DOI: 10.1016/j.biochi.2006.10.009
卷: 89, 期:1, 页:169-177
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 2;4
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Endothelial nitric oxide synthase (eNOS) plays a crucial role in the regulation of a variety of cardiovascular functions. Many studies have shown that dietary n-3 polyunsaturated fatty acids (PUFAs) have beneficial effects on coronary atherosclerosis. However, the mechanisms of n-3 PUFAs regulation in eNOS activation remain unknown. In the present study we investigated the effects of eicosapentaenoic acid (EPA, 20:5 n-3) on subcellular distribution of eNOS and lipid composition of caveolae. We demonstrated for the first time that EPA treatment profoundly altered lipid composition and fatty acyl substitutions of phospholipids in caveolae. We found that caveolin-1 was solely located in caveolae fractions in control cells, and EPA treatment displaced caveolin-1 from caveolae. eNOS was detected in the caveolin-enriched fractions and noncaveolae fractions in control cells. EPA treatment induced the translocation of eNOS from caveolae fractions to soluble fractions. P-eNOS was also distributed in both fractions. After EPA treatment, the level of p-eNOS in each fraction was increased but the distribution of which was unaffected. Moreover, the results of immunofluorescence confirmed that EPA could redistribute caveolin-1 and eNOS in plasma membrane. eNOS activity in HUVEC cells was increased after EPA treatment, which was in a dose dependent manner. And incubation with 50 mu M EPA had the maximum effect on eNOS activity. Our results suggested that eNOS translocation was paralleled by a stimulated capacity for NO production in the cells. We found that total Akt and p-Akt were primarily presented in heavy membranes in control cells, and the relative level of p-Akt increased but the distribution did not change after EPA treatment. The distribution of CaM was slightly changed after EPA treatment.
关键词[WOS]: PROTEIN-KINASE B ;  LOCALIZATION ;  PHOSPHORYLATION ;  ACTIVATION ;  RAFTS ;  OMEGA-3-FATTY-ACIDS ;  ATHEROSCLEROSIS ;  MICRODOMAINS ;  MEMBRANES ;  SYSTEM
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000243630100015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98777
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Jinling Hosp, Inst Gen Surg, Nanjing 210002, Peoples R China
2.Chinese Acad Sci, Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
3.Nanjing Univ, Sch Med, Nanjing 210093, Peoples R China

Recommended Citation:
Li, Qiurong,Zhang, Qiang,Wang, Meng,et al. Eicosapentaenoic acid modifies lipid composition in caveolae and induces translocation of endothelial nitric oxide synthase[J]. BIOCHIMIE,2007,89(1):169-177.
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