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题名: Parallel microfluidic networks for studying cellular response to chemical modulation
作者: Liu, Dayu;  Wang, Lihui;  Zhong, Runtao;  Li, Bowei;  Ye, Nannan;  Liu, Xin;  Lin, Bingcheng
通讯作者: 刘大渔 ;  林炳承
关键词: microfluidic chip ;  gradient concentration ;  glutathione ;  drug resistance
刊名: JOURNAL OF BIOTECHNOLOGY
发表日期: 2007-09-15
DOI: 10.1016/j.jbiotec.2007.06.014
卷: 131, 期:3, 页:286-292
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 3;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: A microfluidic chip featuring parallel gradient-generating networks etched on glass plate was designed and fabricated. The dam and weir structures were fabricated to facilitate cell positioning and seeding, respectively. The microchip contains five gradient generators and 30 cell chambers where the resulted concentration gradients of drugs are delivered to stimulate the on-chip cultured cells. This microfluidics exploits the advantage of lab-on-a-chip technology by integrating the generation of drug concentration gradients and a series of cell operations including seeding, culture, stimulation and staining into a chip. Steady parallel concentration gradients were generated by flowing two fluids in each network. The microchip described above was applied in studying the role of reduced glutathione (GSH) in MCF-7 cells' chemotherapy sensitivity. The parental breast cancer cell line, MCF-7 and the derived adriamycin resistant cell line MCF-7(adm) were treated with concentration gradients of arsenic trioxide (ATO) and N-acetyl cysteine (NAC) for GSH modulation, followed by exposure to adriamycin. The intracellular GSH level and cell viability were assessed by fluorescence image analysis. GSH levels of both cell lines were down-regulated upon ATO treatment and up-regulated upon NAC treatment. For both cell lines, suppression of intracellular GSH by treatment with ATO has been shown to increase chemotherapy sensitivity; conversely, elevation of intracellular GSH by treatment with NAC leads to increased drug resistance. The results indicated that high intracellular GSH level has negative effect on chemotherapy sensitivity, while depletion of cellular GSH may serve as an effective way to improve chemotherapy sensitivity. The integrated microfluidic chip is able to perform multiparametric pharmacological profiling with easy operation, thus, holds great potential for extrapolation to the high-content drug screening. (C) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: DRUG-RESISTANCE ;  GLUTATHIONE ;  INDUCTION ;  CELLS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000250031300010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98891
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Improve Med Instruments Co Ltd, Dept Biomed Engn Ctr, Guangzhou 510370, Guangdong, Peoples R China
2.Dalian Med Univ, Dept Pathol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Liu, Dayu,Wang, Lihui,Zhong, Runtao,et al. Parallel microfluidic networks for studying cellular response to chemical modulation[J]. JOURNAL OF BIOTECHNOLOGY,2007,131(3):286-292.
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