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High performance liquid chromatography-mass spectrometry analysis of protoberberine alkaloids in medicine herbs
Ren, Lingling; Xue, Xingya; Zhang, Feifang; Xu, Qing; Liang, Xinmiao; Liang XM(梁鑫淼); Liang XM(梁鑫淼)
关键词Atmospheric Pressure Chemical Ionization-ms Coptidis Rhizoma Electrospray Ionization-ms High-performance Liquid Chromatography Phellodendri Cortex
刊名JOURNAL OF SEPARATION SCIENCE
2007-04-01
DOI10.1002/jssc.200600246
30期:6页:833-842
收录类别SCI
文章类型Article
部门归属18
项目归属1803
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TRADITIONAL CHINESE MEDICINE ; COPTIDIS RHIZOMA ; BERBERINE ; PALMATINE ; HPLC ; PLASMA ; MS ; EXTRACTION ; COPTISINE
英文摘要RP-HPLC is the main method for the analysis of alkaloids. However, peak tailing is a problem that commonly occurs in the separation of alkaloids. In order to overcome this, three kinds of RP columns were compared for the analysis of protoberberine alkaloids in Coptidis Rhizoma and Phellodendri Cortex in this work. XTerra MS C18 column was the best one which gave the best symmetry factor under the same conditions. With this column, a good separation of the crude extracts of C. Rhizoma and P. Cortex was achieved using 0.1% v/v formic acid buffer and methanol as mobile phase. At the same time, the crude extracts of C. Rhizoma and P. Cortex were analyzed by the LC-ESI-MSn and LC-atmospheric pressure chemical ionization (APCI)-MSn methods. In the analysis of HPLC-ESI/MSn, structures of five protoberberine alkaloids were elucidated, compared to authentic standards, and data from the literature. At the same time, the structure of a novel compound was elucidated. In the HPLC-APCI/MSn analysis, there was an interesting phenomenon that the relative abundance of the ions M+ and [M + 2](+) was different for different alkaloids. The possible fragmentation pathways of protoberberine alkaloids in APCI/MS analysis were studied for the first time in the present work.
语种英语
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WOS记录号WOS:000246348000008
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被引频次:44[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/98907
专题中国科学院大连化学物理研究所
通讯作者Liang XM(梁鑫淼); Liang XM(梁鑫淼)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Liaoning, Peoples R China
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GB/T 7714
Ren, Lingling,Xue, Xingya,Zhang, Feifang,et al. High performance liquid chromatography-mass spectrometry analysis of protoberberine alkaloids in medicine herbs[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(6):833-842.
APA Ren, Lingling.,Xue, Xingya.,Zhang, Feifang.,Xu, Qing.,Liang, Xinmiao.,...&梁鑫淼.(2007).High performance liquid chromatography-mass spectrometry analysis of protoberberine alkaloids in medicine herbs.JOURNAL OF SEPARATION SCIENCE,30(6),833-842.
MLA Ren, Lingling,et al."High performance liquid chromatography-mass spectrometry analysis of protoberberine alkaloids in medicine herbs".JOURNAL OF SEPARATION SCIENCE 30.6(2007):833-842.
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