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题名: High performance liquid chromatography-mass spectrometry analysis of protoberberine alkaloids in medicine herbs
作者: Ren, Lingling;  Xue, Xingya;  Zhang, Feifang;  Xu, Qing;  Liang, Xinmiao
通讯作者: 梁鑫淼
关键词: atmospheric pressure chemical ionization-MS ;  Coptidis rhizoma ;  electrospray ionization-MS ;  high-performance liquid chromatography ;  Phellodendri Cortex
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2007-04-01
DOI: 10.1002/jssc.200600246
卷: 30, 期:6, 页:833-842
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: RP-HPLC is the main method for the analysis of alkaloids. However, peak tailing is a problem that commonly occurs in the separation of alkaloids. In order to overcome this, three kinds of RP columns were compared for the analysis of protoberberine alkaloids in Coptidis Rhizoma and Phellodendri Cortex in this work. XTerra MS C18 column was the best one which gave the best symmetry factor under the same conditions. With this column, a good separation of the crude extracts of C. Rhizoma and P. Cortex was achieved using 0.1% v/v formic acid buffer and methanol as mobile phase. At the same time, the crude extracts of C. Rhizoma and P. Cortex were analyzed by the LC-ESI-MSn and LC-atmospheric pressure chemical ionization (APCI)-MSn methods. In the analysis of HPLC-ESI/MSn, structures of five protoberberine alkaloids were elucidated, compared to authentic standards, and data from the literature. At the same time, the structure of a novel compound was elucidated. In the HPLC-APCI/MSn analysis, there was an interesting phenomenon that the relative abundance of the ions M+ and [M + 2](+) was different for different alkaloids. The possible fragmentation pathways of protoberberine alkaloids in APCI/MS analysis were studied for the first time in the present work.
关键词[WOS]: TRADITIONAL CHINESE MEDICINE ;  COPTIDIS RHIZOMA ;  BERBERINE ;  PALMATINE ;  HPLC ;  PLASMA ;  MS ;  EXTRACTION ;  COPTISINE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000246348000008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/98907
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Ren, Lingling,Xue, Xingya,Zhang, Feifang,et al. High performance liquid chromatography-mass spectrometry analysis of protoberberine alkaloids in medicine herbs[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(6):833-842.
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