DICP OpenIR
Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics
Jiang, Xinning; Jiang, Xiaogang; Han, Guanghui; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
Source PublicationBMC BIOINFORMATICS
2007-08-31
ISSN1471-2105
DOI10.1186/1471-2105-8-323
Volume8Issue:323Pages:40190
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology ; Mathematical & Computational Biology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Mathematical & Computational Biology
WOS KeywordTANDEM MASS-SPECTROMETRY ; IDENTIFICATION TECHNOLOGY ; LIQUID-CHROMATOGRAPHY ; IDENTIFYING PROTEINS ; STATISTICAL-MODEL ; YEAST PROTEOME ; SPECTRAL DATA ; IN-VIVO ; PEPTIDE ; MS/MS
AbstractBackground: In proteomic analysis, MS/MS spectra acquired by mass spectrometer are assigned to peptides by database searching algorithms such as SEQUEST. The assignations of peptides to MS/ MS spectra by SEQUEST searching algorithm are defined by several scores including Xcorr, Delta Cn, Sp, Rsp, matched ion count and so on. Filtering criterion using several above scores is used to isolate correct identifications from random assignments. However, the filtering criterion was not favorably optimized up to now.
Language英语
URL查看原文
WOS IDWOS:000250595500001
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/99011
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Xinning,Jiang, Xiaogang,Han, Guanghui,et al. Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics[J]. BMC BIOINFORMATICS,2007,8(323):40190.
APA Jiang, Xinning.,Jiang, Xiaogang.,Han, Guanghui.,Ye, Mingliang.,Zou, Hanfa.,...&邹汉法.(2007).Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics.BMC BIOINFORMATICS,8(323),40190.
MLA Jiang, Xinning,et al."Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics".BMC BIOINFORMATICS 8.323(2007):40190.
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