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Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics
Jiang, Xinning; Jiang, Xiaogang; Han, Guanghui; Ye, Mingliang; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
刊名BMC BIOINFORMATICS
2007-08-31
ISSN1471-2105
DOI10.1186/1471-2105-8-323
8期:323页:40190
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Mathematical & Computational Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Mathematical & Computational Biology
关键词[WOS]TANDEM MASS-SPECTROMETRY ; IDENTIFICATION TECHNOLOGY ; LIQUID-CHROMATOGRAPHY ; IDENTIFYING PROTEINS ; STATISTICAL-MODEL ; YEAST PROTEOME ; SPECTRAL DATA ; IN-VIVO ; PEPTIDE ; MS/MS
英文摘要Background: In proteomic analysis, MS/MS spectra acquired by mass spectrometer are assigned to peptides by database searching algorithms such as SEQUEST. The assignations of peptides to MS/ MS spectra by SEQUEST searching algorithm are defined by several scores including Xcorr, Delta Cn, Sp, Rsp, matched ion count and so on. Filtering criterion using several above scores is used to isolate correct identifications from random assignments. However, the filtering criterion was not favorably optimized up to now.
语种英语
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WOS记录号WOS:000250595500001
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被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/99011
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
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Jiang, Xinning,Jiang, Xiaogang,Han, Guanghui,et al. Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics[J]. BMC BIOINFORMATICS,2007,8(323):40190.
APA Jiang, Xinning.,Jiang, Xiaogang.,Han, Guanghui.,Ye, Mingliang.,Zou, Hanfa.,...&邹汉法.(2007).Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics.BMC BIOINFORMATICS,8(323),40190.
MLA Jiang, Xinning,et al."Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics".BMC BIOINFORMATICS 8.323(2007):40190.
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