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题名: Automated injection of uncleaned samples using a ten-port switching valve and a strong cation-exchange trap column for proteome analysis
作者: Wang, Fangjun;  Jiang, Xiaogang;  Feng, Shun;  Tian, Ruijun;  Jiang, Xinning;  Han, Guanghui;  Liu, Hongwei;  Ye, Mingliang;  Zou, Hanfa
通讯作者: 邹汉法
关键词: mu HPLC-MS/MS ;  shotgun proteome analysis ;  automated sample injection ;  SCX trap column ;  void volume insensitive ;  multidimensional separation
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2007-11-09
DOI: 10.1016/j.chroma.2007.09.048
卷: 1171, 期:1-2, 页:56-62
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (mu HPLC-MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5 mu L on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port switching valve was established for efficient shotgun proteome analysis. In this system, a nanoflow switching valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis. (c) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  MONOLITH CAPILLARY COLUMN ;  SHOTGUN PROTEOMICS ;  IONIZATION EMITTER ;  IDENTIFICATION ;  PEPTIDES ;  SYSTEM ;  PHASE ;  MS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000250793400008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99043
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograp R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Fangjun,Jiang, Xiaogang,Feng, Shun,et al. Automated injection of uncleaned samples using a ten-port switching valve and a strong cation-exchange trap column for proteome analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2007,1171(1-2):56-62.
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