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Differential role of microenvironment in microencapsulation for improved cell tolerance to stress
Sun, Zhi-jie; Lv, Guo-jun; Li, Shuang-yue; Yu, Wei-ting; Wang, Wei; Xie, Yu-bing; Ma, Xiaojun; Ma XJ(马小军); Ma XJ(马小军)
关键词Sacchromyces Cerevisiae Aca Microcapsules Microenvironment Stress Tolerance
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
2007-07-01
DOI10.1007/s00253-007-0960-6
75期:6页:1419-1427
收录类别SCI
文章类型Article
部门归属18
项目归属1802
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]IMMOBILIZED SACCHAROMYCES-CEREVISIAE ; FERMENTATION PROPERTIES ; ETHANOL TOLERANCE ; ESCHERICHIA-COLI ; MICROBIAL-GROWTH ; OSMOTIC-STRESS ; YEAST ; MICROCAPSULES ; MEMBRANES ; SURVIVAL
英文摘要The effect of the microenvironment in alginate-chitosan-alginate (ACA) microcapsules with liquid core (LCM) and solid core (SCM) on the physiology and stress tolerance of Sacchromyces cerevisiae was studied. The suspended cells were used as control. Cells cultured in liquid core microcapsules showed a nearly twofold increase in the intracellular glycerol content, trehalose content, and the superoxide dismutase (SOD) activity, which are stress tolerance substances, while SCM did not cause the significant physiological variation. In accordance with the physiological modification after being challenged with osmotic stress (NaCl), oxidative stress (H(2)O(2)), ethanol stress, and heat shock stress, the cell survival in LCM was increased. However, SCM can only protect the cells from damaging under ethanol stress. Cells released from LCM were more resistant to hyperosmotic stress, oxidative stress, and heat shock stress than cells liberated from SCM. Based on reasonable analysis, a method was established to estimate the effect of microenvironment of LCM and SCM on the protection of cells against stress factors. It was found that the resistance of LCM to hyperosmotic stress, oxidative stress, and heat shock stress mainly depend on the domestication effect of LCM's microenvironment. The physical barrier of LCM constituted by alginate-chitosan membrane and liquid alginate matrix separated the cells from the damage of oxidative stress and ethanol stress. The significant tolerance against ethanol stress of SCM attributed to the physical barrier consists of solid alginate-calcium matrix and alginate-chitosan membrane.
语种英语
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WOS记录号WOS:000247463100024
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被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/99047
专题中国科学院大连化学物理研究所
通讯作者Ma XJ(马小军); Ma XJ(马小军)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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GB/T 7714
Sun, Zhi-jie,Lv, Guo-jun,Li, Shuang-yue,et al. Differential role of microenvironment in microencapsulation for improved cell tolerance to stress[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,75(6):1419-1427.
APA Sun, Zhi-jie.,Lv, Guo-jun.,Li, Shuang-yue.,Yu, Wei-ting.,Wang, Wei.,...&马小军.(2007).Differential role of microenvironment in microencapsulation for improved cell tolerance to stress.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,75(6),1419-1427.
MLA Sun, Zhi-jie,et al."Differential role of microenvironment in microencapsulation for improved cell tolerance to stress".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 75.6(2007):1419-1427.
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