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题名: Differential role of microenvironment in microencapsulation for improved cell tolerance to stress
作者: Sun, Zhi-jie;  Lv, Guo-jun;  Li, Shuang-yue;  Yu, Wei-ting;  Wang, Wei;  Xie, Yu-bing;  Ma, Xiaojun
通讯作者: 马小军
关键词: Sacchromyces cerevisiae ;  ACA microcapsules ;  microenvironment ;  stress tolerance
刊名: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
发表日期: 2007-07-01
DOI: 10.1007/s00253-007-0960-6
卷: 75, 期:6, 页:1419-1427
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1802
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: The effect of the microenvironment in alginate-chitosan-alginate (ACA) microcapsules with liquid core (LCM) and solid core (SCM) on the physiology and stress tolerance of Sacchromyces cerevisiae was studied. The suspended cells were used as control. Cells cultured in liquid core microcapsules showed a nearly twofold increase in the intracellular glycerol content, trehalose content, and the superoxide dismutase (SOD) activity, which are stress tolerance substances, while SCM did not cause the significant physiological variation. In accordance with the physiological modification after being challenged with osmotic stress (NaCl), oxidative stress (H(2)O(2)), ethanol stress, and heat shock stress, the cell survival in LCM was increased. However, SCM can only protect the cells from damaging under ethanol stress. Cells released from LCM were more resistant to hyperosmotic stress, oxidative stress, and heat shock stress than cells liberated from SCM. Based on reasonable analysis, a method was established to estimate the effect of microenvironment of LCM and SCM on the protection of cells against stress factors. It was found that the resistance of LCM to hyperosmotic stress, oxidative stress, and heat shock stress mainly depend on the domestication effect of LCM's microenvironment. The physical barrier of LCM constituted by alginate-chitosan membrane and liquid alginate matrix separated the cells from the damage of oxidative stress and ethanol stress. The significant tolerance against ethanol stress of SCM attributed to the physical barrier consists of solid alginate-calcium matrix and alginate-chitosan membrane.
关键词[WOS]: IMMOBILIZED SACCHAROMYCES-CEREVISIAE ;  FERMENTATION PROPERTIES ;  ETHANOL TOLERANCE ;  ESCHERICHIA-COLI ;  MICROBIAL-GROWTH ;  OSMOTIC-STRESS ;  YEAST ;  MICROCAPSULES ;  MEMBRANES ;  SURVIVAL
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000247463100024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99047
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Sun, Zhi-jie,Lv, Guo-jun,Li, Shuang-yue,et al. Differential role of microenvironment in microencapsulation for improved cell tolerance to stress[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,75(6):1419-1427.
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