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Differential role of microenvironment in microencapsulation for improved cell tolerance to stress
Sun, Zhi-jie; Lv, Guo-jun; Li, Shuang-yue; Yu, Wei-ting; Wang, Wei; Xie, Yu-bing; Ma, Xiaojun; Ma XJ(马小军); Ma XJ(马小军)
KeywordSacchromyces Cerevisiae Aca Microcapsules Microenvironment Stress Tolerance
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
2007-07-01
DOI10.1007/s00253-007-0960-6
Volume75Issue:6Pages:1419-1427
Indexed BySCI
SubtypeArticle
Department18
Funding Project1802
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordIMMOBILIZED SACCHAROMYCES-CEREVISIAE ; FERMENTATION PROPERTIES ; ETHANOL TOLERANCE ; ESCHERICHIA-COLI ; MICROBIAL-GROWTH ; OSMOTIC-STRESS ; YEAST ; MICROCAPSULES ; MEMBRANES ; SURVIVAL
AbstractThe effect of the microenvironment in alginate-chitosan-alginate (ACA) microcapsules with liquid core (LCM) and solid core (SCM) on the physiology and stress tolerance of Sacchromyces cerevisiae was studied. The suspended cells were used as control. Cells cultured in liquid core microcapsules showed a nearly twofold increase in the intracellular glycerol content, trehalose content, and the superoxide dismutase (SOD) activity, which are stress tolerance substances, while SCM did not cause the significant physiological variation. In accordance with the physiological modification after being challenged with osmotic stress (NaCl), oxidative stress (H(2)O(2)), ethanol stress, and heat shock stress, the cell survival in LCM was increased. However, SCM can only protect the cells from damaging under ethanol stress. Cells released from LCM were more resistant to hyperosmotic stress, oxidative stress, and heat shock stress than cells liberated from SCM. Based on reasonable analysis, a method was established to estimate the effect of microenvironment of LCM and SCM on the protection of cells against stress factors. It was found that the resistance of LCM to hyperosmotic stress, oxidative stress, and heat shock stress mainly depend on the domestication effect of LCM's microenvironment. The physical barrier of LCM constituted by alginate-chitosan membrane and liquid alginate matrix separated the cells from the damage of oxidative stress and ethanol stress. The significant tolerance against ethanol stress of SCM attributed to the physical barrier consists of solid alginate-calcium matrix and alginate-chitosan membrane.
Language英语
URL查看原文
WOS IDWOS:000247463100024
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/99047
Collection中国科学院大连化学物理研究所
Corresponding AuthorMa XJ(马小军); Ma XJ(马小军)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Sci & Technol, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Sun, Zhi-jie,Lv, Guo-jun,Li, Shuang-yue,et al. Differential role of microenvironment in microencapsulation for improved cell tolerance to stress[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,75(6):1419-1427.
APA Sun, Zhi-jie.,Lv, Guo-jun.,Li, Shuang-yue.,Yu, Wei-ting.,Wang, Wei.,...&马小军.(2007).Differential role of microenvironment in microencapsulation for improved cell tolerance to stress.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,75(6),1419-1427.
MLA Sun, Zhi-jie,et al."Differential role of microenvironment in microencapsulation for improved cell tolerance to stress".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 75.6(2007):1419-1427.
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