中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Specific capture of phosphopeptides by Zr4+-modified monolithic capillary column
作者: Dong, Jing;  Zhou, Houjiang;  Wu, Renan;  Ye, Mingliang;  Zou, Hanfa
通讯作者: 邹汉法
关键词: monolithic capillary column ;  phosphoproteome analysis ;  zirconium phosphate
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2007-11-01
DOI: 10.1002/jssc.200700350
卷: 30, 期:17, 页:2917-2923
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A method to prepare zirconium phosphate (ZrP)-modified monolithic capillary column for highly specific capture of phosphopeptides is presented. In this method, the phosphate monolithic capillary column was prepared by direct copolymerization of the functional monomer containing phosphate group (ethylene glycol methacrylate phosphate) and cross-linker (bis-acrylamide) in a ternary porogenic solvent. Copolymerization of cross-linker and functional monomer simplifies the procedure for the preparation of phosphate chromatographic media. After Zr4+ was immobilized, the ZrP-modified monolithic capillary column was evaluated by the analysis of standard phosphoproteins and the excellent selectivity of this approach was demonstrated by analyzing phosphopeptides in the digest mixture of P-casein and BSA with molar ratio of 1:200.
关键词[WOS]: PHOSPHOPROTEOME ANALYSIS ;  AFFINITY-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  LIQUID-CHROMATOGRAPHY ;  POLYMER MONOLITHS ;  ZIRCONIUM ;  ENRICHMENT ;  PEPTIDES ;  ELECTROCHROMATOGRAPHY ;  SEPARATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000251461500016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99355
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Dong, Jing,Zhou, Houjiang,Wu, Renan,et al. Specific capture of phosphopeptides by Zr4+-modified monolithic capillary column[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(17):2917-2923.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Dong, Jing]'s Articles
 [Zhou, Houjiang]'s Articles
 [Wu, Renan]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Dong, Jing]‘s Articles
 [Zhou, Houjiang]‘s Articles
 [Wu, Renan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace