DICP OpenIR
Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography
Wang, Yun1,2,3; Kong, Liang4; Hu, Lianghai3; Lei, Xiaoyuan3; Yang, Li1; Chou, Guixin1; Zou, Hanfa3; Wang, Changhong1; Bligh, S. W. Annie1; Wang, Zhengtao1,2; Zou HF(邹汉法); Zhengtao Wang; Zou HF(邹汉法); Zhengtao Wang
关键词Comprehensive Two-dimensional Biochromatography Longdan Xiegan Decoction Hsa Column Biological Fingerprinting Analysis Traditional Chinese Medicine
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2007-12-15
ISSN1570-0232
DOI10.1016/j.jchromb.2007.10.027
860期:2页:185-194
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名2;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]2-DIMENSIONAL LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; ANGELICA-SINENSIS ; SEPARATION ; ENANTIOMERS ; GENIPOSIDE ; EXTRACT ; PROTEIN ; PLASMA ; SYSTEM
英文摘要A comprehensive two-dimensional biochromatography method using a silica-bonded human serum albumin (HSA) column and a RP-HPLC column was developed for the biological fingerprinting analysis of bioactive components in a traditional Chinese medicine (TCM) prescription, Longdan Xiegan Decoction (LXD). The biochromatography with HSA-immobilized stationary phase was applied to study the interaction of multiple components in LXD with HSA in the first dimension, and fractions of HSA column were further separated by a silica monolithic ODS column (on-line)/an ODS column (off-line) coupled with a diode array detector and an atmospheric pressure chemical ionization mass spectrometer (APCI-MS). More than 100 compounds in LXD that interacted with the immobilized HSA were separated and analyzed. Among them 19 compounds were identified based on their retention values, UV spectra, molecular weights and mass spectra. The results show that the developed comprehensive two-dimensional biochromatography system reported here is capable of being used for biological fingerprinting analysis of natural products in complex matrices such as extracts of TCMs and their prescriptions. (C) 2007 Elsevier B.V. All rights reserved.
语种英语
原文出处查看原文
WOS记录号WOS:000251919400007
引用统计
被引频次:46[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/99375
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zhengtao Wang; Zou HF(邹汉法); Zhengtao Wang
作者单位1.Shanghai Univ Tradit Chinese Med, Minist Educ Res, Key Lab Stand Chinese Med, Shanghai 201203, Peoples R China
2.China Pharmaceut Univ, Dept Pharmacol, Nanjing 210038, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China
4.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Wang, Yun,Kong, Liang,Hu, Lianghai,et al. Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,860(2):185-194.
APA Wang, Yun.,Kong, Liang.,Hu, Lianghai.,Lei, Xiaoyuan.,Yang, Li.,...&Zhengtao Wang.(2007).Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,860(2),185-194.
MLA Wang, Yun,et al."Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 860.2(2007):185-194.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Yun]的文章
[Kong, Liang]的文章
[Hu, Lianghai]的文章
百度学术
百度学术中相似的文章
[Wang, Yun]的文章
[Kong, Liang]的文章
[Hu, Lianghai]的文章
必应学术
必应学术中相似的文章
[Wang, Yun]的文章
[Kong, Liang]的文章
[Hu, Lianghai]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。