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题名: Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography
作者: Wang, Yun1, 2, 3;  Kong, Liang4;  Hu, Lianghai3;  Lei, Xiaoyuan3;  Yang, Li1;  Chou, Guixin1;  Zou, Hanfa3;  Wang, Changhong1;  Bligh, S. W. Annie1;  Wang, Zhengtao1, 2
通讯作者: 邹汉法 ;  Zhengtao Wang
关键词: comprehensive two-dimensional biochromatography ;  Longdan Xiegan Decoction ;  HSA column ;  biological fingerprinting analysis ;  traditional chinese ;  medicine
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2007-12-15
DOI: 10.1016/j.jchromb.2007.10.027
卷: 860, 期:2, 页:185-194
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 2;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A comprehensive two-dimensional biochromatography method using a silica-bonded human serum albumin (HSA) column and a RP-HPLC column was developed for the biological fingerprinting analysis of bioactive components in a traditional Chinese medicine (TCM) prescription, Longdan Xiegan Decoction (LXD). The biochromatography with HSA-immobilized stationary phase was applied to study the interaction of multiple components in LXD with HSA in the first dimension, and fractions of HSA column were further separated by a silica monolithic ODS column (on-line)/an ODS column (off-line) coupled with a diode array detector and an atmospheric pressure chemical ionization mass spectrometer (APCI-MS). More than 100 compounds in LXD that interacted with the immobilized HSA were separated and analyzed. Among them 19 compounds were identified based on their retention values, UV spectra, molecular weights and mass spectra. The results show that the developed comprehensive two-dimensional biochromatography system reported here is capable of being used for biological fingerprinting analysis of natural products in complex matrices such as extracts of TCMs and their prescriptions. (C) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: 2-DIMENSIONAL LIQUID-CHROMATOGRAPHY ;  TANDEM MASS-SPECTROMETRY ;  ANGELICA-SINENSIS ;  SEPARATION ;  ENANTIOMERS ;  GENIPOSIDE ;  EXTRACT ;  PROTEIN ;  PLASMA ;  SYSTEM
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000251919400007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99375
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanghai Univ Tradit Chinese Med, Minist Educ Res, Key Lab Stand Chinese Med, Shanghai 201203, Peoples R China
2.China Pharmaceut Univ, Dept Pharmacol, Nanjing 210038, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China
4.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Yun,Kong, Liang,Hu, Lianghai,et al. Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,860(2):185-194.
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