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Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography
Wang, Yun1,2,3; Kong, Liang4; Hu, Lianghai3; Lei, Xiaoyuan3; Yang, Li1; Chou, Guixin1; Zou, Hanfa3; Wang, Changhong1; Bligh, S. W. Annie1; Wang, Zhengtao1,2; Zou HF(邹汉法); Zhengtao Wang; Zou HF(邹汉法); Zhengtao Wang
KeywordComprehensive Two-dimensional Biochromatography Longdan Xiegan Decoction Hsa Column Biological Fingerprinting Analysis Traditional Chinese Medicine
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2007-12-15
ISSN1570-0232
DOI10.1016/j.jchromb.2007.10.027
Volume860Issue:2Pages:185-194
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank2;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS Keyword2-DIMENSIONAL LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; ANGELICA-SINENSIS ; SEPARATION ; ENANTIOMERS ; GENIPOSIDE ; EXTRACT ; PROTEIN ; PLASMA ; SYSTEM
AbstractA comprehensive two-dimensional biochromatography method using a silica-bonded human serum albumin (HSA) column and a RP-HPLC column was developed for the biological fingerprinting analysis of bioactive components in a traditional Chinese medicine (TCM) prescription, Longdan Xiegan Decoction (LXD). The biochromatography with HSA-immobilized stationary phase was applied to study the interaction of multiple components in LXD with HSA in the first dimension, and fractions of HSA column were further separated by a silica monolithic ODS column (on-line)/an ODS column (off-line) coupled with a diode array detector and an atmospheric pressure chemical ionization mass spectrometer (APCI-MS). More than 100 compounds in LXD that interacted with the immobilized HSA were separated and analyzed. Among them 19 compounds were identified based on their retention values, UV spectra, molecular weights and mass spectra. The results show that the developed comprehensive two-dimensional biochromatography system reported here is capable of being used for biological fingerprinting analysis of natural products in complex matrices such as extracts of TCMs and their prescriptions. (C) 2007 Elsevier B.V. All rights reserved.
Language英语
URL查看原文
WOS IDWOS:000251919400007
Citation statistics
Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/99375
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zhengtao Wang; Zou HF(邹汉法); Zhengtao Wang
Affiliation1.Shanghai Univ Tradit Chinese Med, Minist Educ Res, Key Lab Stand Chinese Med, Shanghai 201203, Peoples R China
2.China Pharmaceut Univ, Dept Pharmacol, Nanjing 210038, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116023, Peoples R China
4.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yun,Kong, Liang,Hu, Lianghai,et al. Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,860(2):185-194.
APA Wang, Yun.,Kong, Liang.,Hu, Lianghai.,Lei, Xiaoyuan.,Yang, Li.,...&Zhengtao Wang.(2007).Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,860(2),185-194.
MLA Wang, Yun,et al."Biological fingerprinting analysis of the traditional Chinese prescription Longdan Xiegan Decoction by on/off-line comprehensive two-dimensional biochromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 860.2(2007):185-194.
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