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题名: Purification and biochemical characterization of soluble methane monooxygenase hydroxylase from Methylosinus trichosporium IMV 3011
作者: Hua Shaofeng;  Li Shuben;  Xin Jiayin;  Niu Jianzhong;  Xia Chungu;  Tan Haidong;  Tang Wei
通讯作者: Li Shuben
关键词: characterization ;  hydroxylase ;  phylogenetic analysis ;  purification ;  methane monooxygenases
刊名: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
发表日期: 2007
DOI: 10.1271/bbb.60402
卷: 71, 期:1, 页:122-129
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 2;6
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Chemistry, Applied ;  Food Science & Technology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Food Science & Technology
英文摘要: Methane monooxygenase hydroxylase was purified by chromatography and characterized by electrophoresis and spectroscopy. The molecular mass of hydroxylase was 201.3 KDa as determined by gel filtration, whereas the total molecular mass was 234 KDa as judged by SDS-PAGE. Structure study indicated that the enzyme is a homodimer structure, consisting of three subunits, designated alpha, beta, and gamma, with molecular masses of 58 KDa, 36 KDa, and 23 KDa respectively. IEF analysis indicated that the enzyme has a pI of 5.2. The UV-Vis spectrum of hydroxylase revealed an absorption peak near 281 run and a weak shoulder peak around 395 nm-420 nm, and a fluorescence spectrum revealed an emission peak at 341.3 nm. Circular dichroism measurement indicated that hydroxylase mainly consists of alpha-helical regions. Finally, phylogenetic analysis indicated that this strain is very close to Methylosinus trichosporium OB3b.
关键词[WOS]: METHYLOCOCCUS-CAPSULATUS-BATH ;  METHANOTROPHIC BACTERIA ;  CRYSTAL-STRUCTURE ;  COMPONENT ;  OXIDATION ;  TRICHLOROETHYLENE ;  OB3B ;  GYJ3
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000243915600016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99387
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Oxo Synthesis & Select Oxidat, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Hua Shaofeng,Li Shuben,Xin Jiayin,et al. Purification and biochemical characterization of soluble methane monooxygenase hydroxylase from Methylosinus trichosporium IMV 3011[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2007,71(1):122-129.
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