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题名: Molecular sequencing and analysis of soluble methane monooxygenase gene clusters from methanotroph Methylomonas sp. GYJ3
作者: Hua, Shao-feng;  Li, Shu-ben;  Tan, Hai-dong
通讯作者: Shu-ben Li
关键词: electron microscopy ;  phylogenetic analysis ;  sequence analysis ;  soluble methane monooxygenase ;  16S rDNA
刊名: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2007-03-01
DOI: 10.1007/s11274-006-9227-0
卷: 23, 期:3, 页:323-330
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 2;3
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: A methanotroph Methylomonas sp. GYJ3 was isolated, whose sMMO genes and 16S rDNA were sequenced and analysed, demonstrating that the bacterium might be a type I methanotroph (gamma-Proteobacteria) and was closer to Methylomonas sp. KSWIII/KSPIII. This result was consistent with the result previously determined by biochemistry and morphological taxonomy. Sequence comparison among six open reading frames and the deduced amino acid sequences of the sMMO genes from six strains revealed that the strain GYJ3 had highly conserved regions in MMOX with other strains, amounting to 78-99% homology at protein level and 71-97% homology at DNA level. Highly conserved sequences lay in two iron-binding regions. Furthermore, scanning electron microscopy of the strain GYJ3 showed rod shapes with a slightly bent configuration on the even surfaces and with plump bodies.
关键词[WOS]: METHYLOSINUS-TRICHOSPORIUM OB3B ;  METHYLOCOCCUS-CAPSULATUS BATH ;  HYDROXYLASE COMPONENT ;  CRYSTAL-STRUCTURE ;  PROTEIN-B ;  TRICHLOROETHYLENE ;  BACTERIA ;  EXPRESSION ;  OXIDATION ;  DEGRADATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000244323200003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99389
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, State Key Lab Oxo Synth & Select Oxidat, Lanzhou 730000, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Hua, Shao-feng,Li, Shu-ben,Tan, Hai-dong. Molecular sequencing and analysis of soluble methane monooxygenase gene clusters from methanotroph Methylomonas sp. GYJ3[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2007,23(3):323-330.
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