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题名: Screening of phage displayed human liver cDNA library against dexamethasone
作者: Yu, Xiaoming1, 2;  Zhao, Peng1, 2;  Zhang, Weibing1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: phage display ;  cDNA library ;  dexamethasone ;  interaction
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2007-12-21
DOI: 10.1016/j.jpba.2007.09.008
卷: 45, 期:5, 页:701-705
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: This paper described an attempt to establish a new method to screen the target biomolecule from phage displayed cDNA library against small molecule drug insoluble in water. Dexamethasone was selected as the model drug, and the screening was carried out in an Eppendorf tube packed with the drug. The whole procedure was monitored by PCR with the enriched specific phage clone as the template. After four rounds screening, the PCR products of selected phages with the lengths of 400 and 600 bp were sequenced, and revealed identical sequences with cytochrome c oxidase subunit III and albumin respectively by GenBank searching. Furthermore, frontal analysis-capillary electrophoresis (FA-CE) was performed to study the interaction between dexamethasone and albumin, and the binding constant was calculated to be 1.153 x 10(3), validating the weak specific interaction between the drug and the target protein. All these results demonstrated that with insoluble drug as the solid phase directly, the screening of target large molecule expressed in phage display cDNA library was feasible, which might pave an easy way to screen the candidate drug targets. (c) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  PROTEIN INTERACTIONS ;  BINDING-PROTEIN ;  EXPRESSION ;  IDENTIFICATION ;  SELECTION ;  CLONING ;  SURFACE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000252013300001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99465
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Yu, Xiaoming,Zhao, Peng,Zhang, Weibing,et al. Screening of phage displayed human liver cDNA library against dexamethasone[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,45(5):701-705.
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