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Screening of phage displayed human liver cDNA library against dexamethasone
Yu, Xiaoming1,2; Zhao, Peng1,2; Zhang, Weibing1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华); Zhang LH(张丽华)
关键词Phage Display Cdna Library Dexamethasone Interaction
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2007-12-21
DOI10.1016/j.jpba.2007.09.008
45期:5页:701-705
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]CAPILLARY-ELECTROPHORESIS ; PROTEIN INTERACTIONS ; BINDING-PROTEIN ; EXPRESSION ; IDENTIFICATION ; SELECTION ; CLONING ; SURFACE
英文摘要This paper described an attempt to establish a new method to screen the target biomolecule from phage displayed cDNA library against small molecule drug insoluble in water. Dexamethasone was selected as the model drug, and the screening was carried out in an Eppendorf tube packed with the drug. The whole procedure was monitored by PCR with the enriched specific phage clone as the template. After four rounds screening, the PCR products of selected phages with the lengths of 400 and 600 bp were sequenced, and revealed identical sequences with cytochrome c oxidase subunit III and albumin respectively by GenBank searching. Furthermore, frontal analysis-capillary electrophoresis (FA-CE) was performed to study the interaction between dexamethasone and albumin, and the binding constant was calculated to be 1.153 x 10(3), validating the weak specific interaction between the drug and the target protein. All these results demonstrated that with insoluble drug as the solid phase directly, the screening of target large molecule expressed in phage display cDNA library was feasible, which might pave an easy way to screen the candidate drug targets. (c) 2007 Elsevier B.V. All rights reserved.
语种英语
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WOS记录号WOS:000252013300001
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/99465
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华); Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Yu, Xiaoming,Zhao, Peng,Zhang, Weibing,et al. Screening of phage displayed human liver cDNA library against dexamethasone[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,45(5):701-705.
APA Yu, Xiaoming.,Zhao, Peng.,Zhang, Weibing.,Zhang, Lihua.,Zhang, Yukui.,...&张丽华.(2007).Screening of phage displayed human liver cDNA library against dexamethasone.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,45(5),701-705.
MLA Yu, Xiaoming,et al."Screening of phage displayed human liver cDNA library against dexamethasone".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 45.5(2007):701-705.
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