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题名: Cell-based high content screening using an integrated microfluidic device
作者: Ye, Nannan;  Qin, Jianhua;  Shi, Weiwei;  Liu, Xin;  Lin, Bingcheng
通讯作者: 林炳承 ;  秦建华
刊名: LAB ON A CHIP
发表日期: 2007
DOI: 10.1039/b711513j
卷: 7, 期:12, 页:1696-1704
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: High content screening (HCS) has quickly established itself as a core technique in the early stage of drug discovery for secondary compound screening. It allows several independent cellular parameters to be measured in a single cell or populations of cells in a single assay. In this work, we describe high content screening for the multiparametric measurement of cellular responses in human liver carcinoma (HepG2) cells using an integrated microfluidic device. This device consists of multiple drug gradient generators and parallel cell culture chambers, in which the processes of liquid dilution and diffusion, micro-scale cell culture, cell stimulation and cell labeling can be integrated into a single device. The simple assay provides multiparametric measurements of plasma membrane permeability, nuclear size, mitochondrial transmembrane potential and intracellular redox states in anti-cancer drug-induced apoptosis of HepG2 cells. The established platform is able to rapidly extract the maximum of information from tumor cells in response to several drugs varying in concentration, with minimal sample and less time, which is very useful for basic biomedical research and cancer treatment.
关键词[WOS]: APOPTOSIS ;  SYSTEMS ;  ARRAY ;  ASSAY ;  BIOREACTOR ;  GENERATION ;  GRADIENTS ;  PLATFORM ;  PATHWAY ;  CULTURE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000251121000018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99495
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China

Recommended Citation:
Ye, Nannan,Qin, Jianhua,Shi, Weiwei,et al. Cell-based high content screening using an integrated microfluidic device[J]. LAB ON A CHIP,2007,7(12):1696-1704.
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