DICP OpenIR
Cell-based high content screening using an integrated microfluidic device
Ye, Nannan; Qin, Jianhua; Shi, Weiwei; Liu, Xin; Lin, Bingcheng; Lin BC(林炳承); Qin JH(秦建华); Lin BC(林炳承); Qin JH(秦建华)
刊名LAB ON A CHIP
2007
DOI10.1039/b711513j
7期:12页:1696-1704
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]APOPTOSIS ; SYSTEMS ; ARRAY ; ASSAY ; BIOREACTOR ; GENERATION ; GRADIENTS ; PLATFORM ; PATHWAY ; CULTURE
英文摘要High content screening (HCS) has quickly established itself as a core technique in the early stage of drug discovery for secondary compound screening. It allows several independent cellular parameters to be measured in a single cell or populations of cells in a single assay. In this work, we describe high content screening for the multiparametric measurement of cellular responses in human liver carcinoma (HepG2) cells using an integrated microfluidic device. This device consists of multiple drug gradient generators and parallel cell culture chambers, in which the processes of liquid dilution and diffusion, micro-scale cell culture, cell stimulation and cell labeling can be integrated into a single device. The simple assay provides multiparametric measurements of plasma membrane permeability, nuclear size, mitochondrial transmembrane potential and intracellular redox states in anti-cancer drug-induced apoptosis of HepG2 cells. The established platform is able to rapidly extract the maximum of information from tumor cells in response to several drugs varying in concentration, with minimal sample and less time, which is very useful for basic biomedical research and cancer treatment.
语种英语
原文出处查看原文
WOS记录号WOS:000251121000018
引用统计
被引频次:160[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/99495
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承); Qin JH(秦建华); Lin BC(林炳承); Qin JH(秦建华)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Ye, Nannan,Qin, Jianhua,Shi, Weiwei,et al. Cell-based high content screening using an integrated microfluidic device[J]. LAB ON A CHIP,2007,7(12):1696-1704.
APA Ye, Nannan.,Qin, Jianhua.,Shi, Weiwei.,Liu, Xin.,Lin, Bingcheng.,...&秦建华.(2007).Cell-based high content screening using an integrated microfluidic device.LAB ON A CHIP,7(12),1696-1704.
MLA Ye, Nannan,et al."Cell-based high content screening using an integrated microfluidic device".LAB ON A CHIP 7.12(2007):1696-1704.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ye, Nannan]的文章
[Qin, Jianhua]的文章
[Shi, Weiwei]的文章
百度学术
百度学术中相似的文章
[Ye, Nannan]的文章
[Qin, Jianhua]的文章
[Shi, Weiwei]的文章
必应学术
必应学术中相似的文章
[Ye, Nannan]的文章
[Qin, Jianhua]的文章
[Shi, Weiwei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。