DICP OpenIR
Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis
Long, Zhicheng; Shen, Zheng; Wu, Dapeng; Qin, Jianhua; Lin, Bingcheng; Lin BC(林炳承); Qin JH(秦建华); Lin BC(林炳承); Qin JH(秦建华)
Source PublicationLAB ON A CHIP
2007
DOI10.1039/b711741h
Volume7Issue:12Pages:1819-1824
Indexed BySCI
SubtypeArticle
Department18
Funding Project1807
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS KeywordPOLYMERASE-CHAIN-REACTION ; PRESSURE INJECTION ; SAMPLE PREPARATION ; CHIP ; SEPARATION ; SYSTEMS ; DNA ; POLY(DIMETHYLSILOXANE) ; PRECONCENTRATION ; AMPLIFICATION
AbstractAn integrated microfluidic device was developed for online coupling of solid-phase extraction to microchip electrophoresis ( chip SPE-CE). With a nanoporous membrane sandwiched between two PDMS substrates, SPE preconcentration and electrophoretic separation can be carried out in upper and lower fluidic layers, separately and sequentially. During the SPE process, the thin membrane can act as a fluid isolator to prevent intermixing between two fluidic channels. However, when a pulse voltage is applied, the membrane becomes a gateable interconnect so that a small plug of concentrated analytes can be online injected into the lower channel for subsequent separations. This multilayer design provides a universal solution to online SPE-CE hyphenation. Both electroosmotic flow and hydrodynamic pumps have been adopted for SPE operation. SPE was performed on a 2.5 mm long microcolumn, with two weirs on both sides to retain the C-18-coated silica beads. Rhodamine 123 and FITC-labelled ephedrine were used to test the operational performance of the hyphenation system. High separation efficiency and thousand-fold signal enhancement were achieved.
Language英语
URL查看原文
WOS IDWOS:000251121000035
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/99497
Collection中国科学院大连化学物理研究所
Corresponding AuthorLin BC(林炳承); Qin JH(秦建华); Lin BC(林炳承); Qin JH(秦建华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Long, Zhicheng,Shen, Zheng,Wu, Dapeng,et al. Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis[J]. LAB ON A CHIP,2007,7(12):1819-1824.
APA Long, Zhicheng.,Shen, Zheng.,Wu, Dapeng.,Qin, Jianhua.,Lin, Bingcheng.,...&秦建华.(2007).Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis.LAB ON A CHIP,7(12),1819-1824.
MLA Long, Zhicheng,et al."Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis".LAB ON A CHIP 7.12(2007):1819-1824.
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