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题名: Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis
作者: Long, Zhicheng;  Shen, Zheng;  Wu, Dapeng;  Qin, Jianhua;  Lin, Bingcheng
通讯作者: 林炳承 ;  秦建华
刊名: LAB ON A CHIP
发表日期: 2007
DOI: 10.1039/b711741h
卷: 7, 期:12, 页:1819-1824
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: An integrated microfluidic device was developed for online coupling of solid-phase extraction to microchip electrophoresis ( chip SPE-CE). With a nanoporous membrane sandwiched between two PDMS substrates, SPE preconcentration and electrophoretic separation can be carried out in upper and lower fluidic layers, separately and sequentially. During the SPE process, the thin membrane can act as a fluid isolator to prevent intermixing between two fluidic channels. However, when a pulse voltage is applied, the membrane becomes a gateable interconnect so that a small plug of concentrated analytes can be online injected into the lower channel for subsequent separations. This multilayer design provides a universal solution to online SPE-CE hyphenation. Both electroosmotic flow and hydrodynamic pumps have been adopted for SPE operation. SPE was performed on a 2.5 mm long microcolumn, with two weirs on both sides to retain the C-18-coated silica beads. Rhodamine 123 and FITC-labelled ephedrine were used to test the operational performance of the hyphenation system. High separation efficiency and thousand-fold signal enhancement were achieved.
关键词[WOS]: POLYMERASE-CHAIN-REACTION ;  PRESSURE INJECTION ;  SAMPLE PREPARATION ;  CHIP ;  SEPARATION ;  SYSTEMS ;  DNA ;  POLY(DIMETHYLSILOXANE) ;  PRECONCENTRATION ;  AMPLIFICATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000251121000035
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99497
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Long, Zhicheng,Shen, Zheng,Wu, Dapeng,et al. Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis[J]. LAB ON A CHIP,2007,7(12):1819-1824.
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