DICP OpenIR
Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis
Long, Zhicheng; Shen, Zheng; Wu, Dapeng; Qin, Jianhua; Lin, Bingcheng; Lin BC(林炳承); Qin JH(秦建华); Lin BC(林炳承); Qin JH(秦建华)
刊名LAB ON A CHIP
2007
DOI10.1039/b711741h
7期:12页:1819-1824
收录类别SCI
文章类型Article
部门归属18
项目归属1807
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]POLYMERASE-CHAIN-REACTION ; PRESSURE INJECTION ; SAMPLE PREPARATION ; CHIP ; SEPARATION ; SYSTEMS ; DNA ; POLY(DIMETHYLSILOXANE) ; PRECONCENTRATION ; AMPLIFICATION
英文摘要An integrated microfluidic device was developed for online coupling of solid-phase extraction to microchip electrophoresis ( chip SPE-CE). With a nanoporous membrane sandwiched between two PDMS substrates, SPE preconcentration and electrophoretic separation can be carried out in upper and lower fluidic layers, separately and sequentially. During the SPE process, the thin membrane can act as a fluid isolator to prevent intermixing between two fluidic channels. However, when a pulse voltage is applied, the membrane becomes a gateable interconnect so that a small plug of concentrated analytes can be online injected into the lower channel for subsequent separations. This multilayer design provides a universal solution to online SPE-CE hyphenation. Both electroosmotic flow and hydrodynamic pumps have been adopted for SPE operation. SPE was performed on a 2.5 mm long microcolumn, with two weirs on both sides to retain the C-18-coated silica beads. Rhodamine 123 and FITC-labelled ephedrine were used to test the operational performance of the hyphenation system. High separation efficiency and thousand-fold signal enhancement were achieved.
语种英语
原文出处查看原文
WOS记录号WOS:000251121000035
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/99497
专题中国科学院大连化学物理研究所
通讯作者Lin BC(林炳承); Qin JH(秦建华); Lin BC(林炳承); Qin JH(秦建华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Long, Zhicheng,Shen, Zheng,Wu, Dapeng,et al. Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis[J]. LAB ON A CHIP,2007,7(12):1819-1824.
APA Long, Zhicheng.,Shen, Zheng.,Wu, Dapeng.,Qin, Jianhua.,Lin, Bingcheng.,...&秦建华.(2007).Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis.LAB ON A CHIP,7(12),1819-1824.
MLA Long, Zhicheng,et al."Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis".LAB ON A CHIP 7.12(2007):1819-1824.
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