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题名: Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase
作者: Zheng, Chao;  Liu, Zhaosheng;  Gao, Ruyu;  Zhang, Lihua;  Zhang, Yukui
通讯作者: 张玉奎
关键词: molecularly imprinted polymer ;  capillary electrochromatography ;  monolithic column ;  epitope approach ;  oxytocin
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2007-07-01
DOI: 10.1007/s00216-007-1325-9
卷: 388, 期:5-6, 页:1137-1145
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 4;4
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Using YPLG (Tyr-Pro-Leu-Gly), a tetrapeptide, as the template, an imprinted monolithic column was prepared and applied to the selective recognition of oxytocin based on the epitope approach and capillary electrochromatography (CEC). By optimizing the polymerization solution in terms of functional monomer, cross-linking reagent, porogen, and imprinted template via CEC evaluations of synthesized columns, an imprinted monolith with good recognition capacity (the imprinting factors for YPLG and oxytocin were 4.499 and 4.013, respectively) and high column efficiency (theoretical plates for YPLG and oxytocin were 22,995 plates/m and 16,952 plates/m, respectively) was achieved. In addition, the effects of various experimental parameters on the recognition of oxytocin, including the organic modifier content, the buffer concentration, and the pH value, were studied systematically. Furthermore, a mixture of oxytocin and other proteins was analyzed using this monolithic CEC column, and oxytocin was eluted much more slowly than other large biomolecules, which demonstrated the high selective recognition ability of such an imprinted monolith for oxytocin with PLG (Pro-Leu-Gly) as the epitope.
关键词[WOS]: MOLECULAR RECOGNITION ;  EPITOPE APPROACH ;  PEPTIDES ;  SEPARATION ;  MEDIA ;  RECEPTORS ;  PROTEINS ;  COLUMN ;  ACID
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000247998700019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99527
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R & A Ctr, Dalian 116023, Peoples R China
2.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China
3.Tianjin Med Univ, Coll Pharm, Tianjin 300203, Peoples R China
4.Nankai Univ, Inst Element Organ Chem, State Key Lab Element Organ Chem, Tianjin 300071, Peoples R China

Recommended Citation:
Zheng, Chao,Liu, Zhaosheng,Gao, Ruyu,et al. Recognition of oxytocin by capillary electrochromatography with monolithic tetrapeptide-imprinted polymer used as the stationary phase[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2007,388(5-6):1137-1145.
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