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题名: Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system
作者: Yu, Linfen1;  Shen, Zheng1;  Mo, Jiankun2;  Dong, Xiuling3;  Qin, Jianhua1;  Lin, Bingcheng1
通讯作者: 林炳承 ;  秦建华
关键词: cell electrophoresis ;  K562 cells ;  microfluidic chip ;  multipoint detection
刊名: ELECTROPHORESIS
发表日期: 2007-12-01
DOI: 10.1002/elps.200700313
卷: 28, 期:24, 页:4741-4747
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this work, the electrophoretic mobility (EPM) measurement of individual cells was investigated by a simple on-chip electrophoresis system with LIF multipoint detection. The system enabled the characterization of cell electrophoresis behavior as well as the fluorescence signal from individual cells simultaneously. The measurement yielded the electropherograms of a large number of cells labeled with dye, in which the migration time and migration distance could be obtained easily. The EPM has been demonstrated to be different between the K562 cells and K562 cells treated with anticancer drug arsenic trioxide (AS(2)O(3)). The K562 cells were found to exhibit a lower EPM compared to the cells after drug addition with different concentration. In this preliminary study, over 300 cells could be analyzed within 2 h, demonstrated a much higher analysis throughput compared with traditional methods. The established system is simple and fast, which is expected to be a promising method for evaluating cell surface properties and to be useful in clinical and pharmaceutical applications.
关键词[WOS]: RED-BLOOD-CELLS ;  ARSENIC TRIOXIDE ;  APOPTOSIS ;  ADSORPTION ;  CYTOMETRY ;  MOBILITY ;  FLOW
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000252465600026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99833
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.2nd Peoples Hosp Guangdong Prov, Guangdong, Peoples R China
3.Dalian Third Municipal Hosp, Dalian, Peoples R China

Recommended Citation:
Yu, Linfen,Shen, Zheng,Mo, Jiankun,et al. Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system[J]. ELECTROPHORESIS,2007,28(24):4741-4747.
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