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题名: Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis
作者: Wang, Fangjun1;  Dong, Jing1;  Ye, Mingliang1;  Jiang, Xiaogang1;  Wu, Ren'an1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: biphasic monolithic column ;  shotgun proteome analysis ;  multidimensional separation ;  mu HPLC-MS/MS
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2008
DOI: 10.1021/pr700562b
卷: 7, 期:1, 页:306-310
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: A biphasic monolithic capillary column with 10 cm segment of strong-cation exchange monolith and 65 cm segment of reversed-phase monolith was prepared within a single 100 mu m i.d. capillary. Separation performance of this column was evaluated by a five-cycle online multidimensional separation of 10 mu g tryptic digest of yeast proteins using nanoflow liquid chromatography coupled with tandem mass spectrometry, and it took 12 h for whole separation under the operating pressure only similar to 900 psi. Totally, 780 distinct proteins were positively identified through assignment of 2953 unique peptides at false-positive rate less than 1%. The good separation performance of this biphasic column was largely attributed to the good orthogonality of the strong-cation exchange monolith and reversed-phase monolith for multidimensional separation.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ORGANIC POLYMER MONOLITHS ;  MASS-SPECTROMETRY ;  IDENTIFICATION TECHNOLOGY ;  MEMBRANE-PROTEINS ;  STATIONARY PHASES ;  YEAST PROTEOME ;  TRAP COLUMN ;  PEPTIDES ;  SAMPLES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000252154200036
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99861
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Fangjun,Dong, Jing,Ye, Mingliang,et al. Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis[J]. JOURNAL OF PROTEOME RESEARCH,2008,7(1):306-310.
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