DICP OpenIR
Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis
Wang, Fangjun; Dong, Jing; Ye, Mingliang; Jiang, Xiaogang; Wu, Ren'an; Zou, Hanfa; Zou HF(邹汉法); Zou HF(邹汉法)
KeywordBiphasic Monolithic Column Shotgun Proteome Analysis Multidimensional Separation Mu Hplc-ms/ms
Source PublicationJOURNAL OF PROTEOME RESEARCH
2008
ISSN1535-3893
DOI10.1021/pr700562b
Volume7Issue:1Pages:306-310
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1;1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; ORGANIC POLYMER MONOLITHS ; MASS-SPECTROMETRY ; IDENTIFICATION TECHNOLOGY ; MEMBRANE-PROTEINS ; STATIONARY PHASES ; YEAST PROTEOME ; TRAP COLUMN ; PEPTIDES ; SAMPLES
AbstractA biphasic monolithic capillary column with 10 cm segment of strong-cation exchange monolith and 65 cm segment of reversed-phase monolith was prepared within a single 100 mu m i.d. capillary. Separation performance of this column was evaluated by a five-cycle online multidimensional separation of 10 mu g tryptic digest of yeast proteins using nanoflow liquid chromatography coupled with tandem mass spectrometry, and it took 12 h for whole separation under the operating pressure only similar to 900 psi. Totally, 780 distinct proteins were positively identified through assignment of 2953 unique peptides at false-positive rate less than 1%. The good separation performance of this biphasic column was largely attributed to the good orthogonality of the strong-cation exchange monolith and reversed-phase monolith for multidimensional separation.
Language英语
URL查看原文
WOS IDWOS:000252154200036
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/99861
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wang, Fangjun,Dong, Jing,Ye, Mingliang,et al. Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis[J]. JOURNAL OF PROTEOME RESEARCH,2008,7(1):306-310.
APA Wang, Fangjun.,Dong, Jing.,Ye, Mingliang.,Jiang, Xiaogang.,Wu, Ren'an.,...&邹汉法.(2008).Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis.JOURNAL OF PROTEOME RESEARCH,7(1),306-310.
MLA Wang, Fangjun,et al."Online multidimensional separation with biphasic monolithic capillary column for shotgun proteome analysis".JOURNAL OF PROTEOME RESEARCH 7.1(2008):306-310.
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