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题名: Technologies and methods for sample pretreatment in efficient proteome and peptidome analysis
作者: Jiang, Xiaogang1, 2;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: MS ;  peptidome ;  sample pretreatment
刊名: PROTEOMICS
发表日期: 2008-02-01
卷: 8, 期:4, 页:686-705
收录类别: SCI
文章类型: Review
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Although great progresses have been made in proteomics during the last decade, proteomics is still in its infancy. Extreme complexity of proteome sample and large dynamic range of protein abundance overwhelm the capability of all currently available analytical platforms. Sample pretreatment is a good approach to reduce the complexity of proteome sample and decrease the dynamic range. In this article, we present an overview of different technologies and methods for sample pretreatment in efficient proteome and peptidome analysis. Methods for isolation of rare amino acid-containing peptides, terminal peptides, PTM peptides and endogenous peptides are reviewed. In addition, two automated sample pretreatment technologies, i.e. automated sample injection and on-line digestion, are also covered.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  METAL AFFINITY-CHROMATOGRAPHY ;  CYSTEINE-CONTAINING PEPTIDES ;  N-LINKED GLYCOPROTEINS ;  LOW-ABUNDANCE PEPTIDES ;  EXCHANGE TRAP COLUMN ;  LARGE-SCALE ANALYSIS ;  TOF MS ANALYSIS ;  PHOSPHOPROTEOME ANALYSIS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000253791600006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99915
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Suzhou Univ, Suzhou 215006, Peoples R China

Recommended Citation:
Jiang, Xiaogang,Ye, Mingliang,Zou, Hanfa. Technologies and methods for sample pretreatment in efficient proteome and peptidome analysis[J]. PROTEOMICS,2008,8(4):686-705.
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