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题名: Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins
作者: Cong, Yongzheng1, 2;  Zhang, Lihua1;  Tao, Dingyin1;  Liang, Yu1;  Zhang, Weibing1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: microchip electrophoresis ;  peptides ;  permanent coating ;  separation voltage ;  two dimensional electrophoresis
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2008-02-01
DOI: 10.1002/jssc.200700444
卷: 31, 期:3, 页:588-594
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 1;1
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A two-dimensional capillary electrophoresis platform, combining isoelectric focusing (IEF) and capillary zone electrophoresis (CZE), was established on a microchip with the channel width and depth as 100 mu m and 40 mu m, respectively. With polyacrylamide as permanent coating, EOF in the microchannel, which could impair the separation, was decreased to 3.4 x 10(-9) m(2) . V(-1) . s(-1), about 1/10 of that obtained in the uncoated set-up. During the separation, peptides were first focused by IEF in the first dimensional channel, and then directly driven into the perpendicular channel by controlling the applied voltages, and separated by CZE. Effects of various experimental parameters, including the electric field strength, channel length, and injection frequency from the first to the second dimensional separation channel, were studied. Under optimized condition, the digests of BSA and proteins extracted from E. coli were separated, and a peak capacity of 540 was obtained, which was far greater than that obtained by each single dimensional separation. All these results showed the promise of multidimensional separation on a microchip for the high-throughput and high-resolution analysis of complex samples.
关键词[WOS]: ISOTACHOPHORESIS-ZONE ELECTROPHORESIS ;  ELECTROOSMOTIC FLOW ;  GEL-ELECTROPHORESIS ;  MICROFLUIDIC CHIPS ;  HIGH-EFFICIENCY ;  SEPARATION ;  PRECONCENTRATION ;  CHROMATOGRAPHY ;  SYSTEMS ;  DEVICE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000253689700018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99923
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Cong, Yongzheng,Zhang, Lihua,Tao, Dingyin,et al. Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins[J]. JOURNAL OF SEPARATION SCIENCE,2008,31(3):588-594.
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