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Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins
Cong, Yongzheng1,2; Zhang, Lihua1; Tao, Dingyin1; Liang, Yu1; Zhang, Weibing1; Zhang, Yukui1; Zhang LH(张丽华); Zhang LH(张丽华)
关键词Microchip Electrophoresis Peptides Permanent Coating Separation Voltage Two Dimensional Electrophoresis
刊名JOURNAL OF SEPARATION SCIENCE
2008-02-01
DOI10.1002/jssc.200700444
31期:3页:588-594
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1;1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]ISOTACHOPHORESIS-ZONE ELECTROPHORESIS ; ELECTROOSMOTIC FLOW ; GEL-ELECTROPHORESIS ; MICROFLUIDIC CHIPS ; HIGH-EFFICIENCY ; SEPARATION ; PRECONCENTRATION ; CHROMATOGRAPHY ; SYSTEMS ; DEVICE
英文摘要A two-dimensional capillary electrophoresis platform, combining isoelectric focusing (IEF) and capillary zone electrophoresis (CZE), was established on a microchip with the channel width and depth as 100 mu m and 40 mu m, respectively. With polyacrylamide as permanent coating, EOF in the microchannel, which could impair the separation, was decreased to 3.4 x 10(-9) m(2) . V(-1) . s(-1), about 1/10 of that obtained in the uncoated set-up. During the separation, peptides were first focused by IEF in the first dimensional channel, and then directly driven into the perpendicular channel by controlling the applied voltages, and separated by CZE. Effects of various experimental parameters, including the electric field strength, channel length, and injection frequency from the first to the second dimensional separation channel, were studied. Under optimized condition, the digests of BSA and proteins extracted from E. coli were separated, and a peak capacity of 540 was obtained, which was far greater than that obtained by each single dimensional separation. All these results showed the promise of multidimensional separation on a microchip for the high-throughput and high-resolution analysis of complex samples.
语种英语
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WOS记录号WOS:000253689700018
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被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/99923
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华); Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
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GB/T 7714
Cong, Yongzheng,Zhang, Lihua,Tao, Dingyin,et al. Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins[J]. JOURNAL OF SEPARATION SCIENCE,2008,31(3):588-594.
APA Cong, Yongzheng.,Zhang, Lihua.,Tao, Dingyin.,Liang, Yu.,Zhang, Weibing.,...&张丽华.(2008).Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins.JOURNAL OF SEPARATION SCIENCE,31(3),588-594.
MLA Cong, Yongzheng,et al."Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins".JOURNAL OF SEPARATION SCIENCE 31.3(2008):588-594.
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