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题名: Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column
作者: Jiang, Xiaogang1, 2;  Dong, Jing1;  Wang, Fangjun1;  Feng, Shun1;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 邹汉法
关键词: automation ;  monolithic analytical capillary column ;  nano-LC-MS/MS ;  peptide separation ;  sample introduction
刊名: ELECTROPHORESIS
发表日期: 2008-04-01
DOI: 10.1002/elps.200700513
卷: 29, 期:8, 页:1612-1618
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1;1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: An automated nano-LC-MS/MS platform without trap column was established, which only used a 20 cm lauryl methacrylate-ethylene dimethacrylate (LMA-EDMA) monolithic capillary column to allow preconcentration and separation of peptides. The monolithic column had the advantages of good permeability and low backpressure resulting in higher flow rates for capillary columns. Tryptic digests of bovine albumin and yeast protein extract were tested using the monolithic column system. High proteomic coverage using this approach were demonstrated in this study. Furthermore, peptide samples extracted from mouse liver were separated by using the monolithic column system combined with size-exclusion chromatography prefractionation. This monolithic column system might be a promising alternative for the automated system previously using a trap column for routine proteome and peptide profiling analysis.
关键词[WOS]: EXCHANGE TRAP COLUMN ;  HUMAN SERUM PROTEOME ;  IONIZATION EMITTER ;  IDENTIFICATION ;  PERFORMANCE ;  MS/MS ;  PROTEINS ;  SYSTEM ;  ELECTROCHROMATOGRAPHY ;  PRECONCENTRATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000255703100004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/99997
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Suzhou Univ, Sch Med, Suzhou 215006, Peoples R China

Recommended Citation:
Jiang, Xiaogang,Dong, Jing,Wang, Fangjun,et al. Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column[J]. ELECTROPHORESIS,2008,29(8):1612-1618.
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