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Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column
Jiang, Xiaogang1,2; Dong, Jing1; Wang, Fangjun1; Feng, Shun1; Ye, Mingliang1; Zou, Hanfa1; Zou HF(邹汉法); Zou HF(邹汉法)
关键词Automation Monolithic Analytical Capillary Column Nano-lc-ms/ms Peptide Separation Sample Introduction
刊名ELECTROPHORESIS
2008-04-01
ISSN0173-0835
DOI10.1002/elps.200700513
29期:8页:1612-1618
收录类别SCI
文章类型Article
部门归属18
项目归属1809
产权排名1;1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]EXCHANGE TRAP COLUMN ; HUMAN SERUM PROTEOME ; IONIZATION EMITTER ; IDENTIFICATION ; PERFORMANCE ; MS/MS ; PROTEINS ; SYSTEM ; ELECTROCHROMATOGRAPHY ; PRECONCENTRATION
英文摘要An automated nano-LC-MS/MS platform without trap column was established, which only used a 20 cm lauryl methacrylate-ethylene dimethacrylate (LMA-EDMA) monolithic capillary column to allow preconcentration and separation of peptides. The monolithic column had the advantages of good permeability and low backpressure resulting in higher flow rates for capillary columns. Tryptic digests of bovine albumin and yeast protein extract were tested using the monolithic column system. High proteomic coverage using this approach were demonstrated in this study. Furthermore, peptide samples extracted from mouse liver were separated by using the monolithic column system combined with size-exclusion chromatography prefractionation. This monolithic column system might be a promising alternative for the automated system previously using a trap column for routine proteome and peptide profiling analysis.
语种英语
原文出处查看原文
WOS记录号WOS:000255703100004
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/99997
专题中国科学院大连化学物理研究所
通讯作者Zou HF(邹汉法); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Suzhou Univ, Sch Med, Suzhou 215006, Peoples R China
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GB/T 7714
Jiang, Xiaogang,Dong, Jing,Wang, Fangjun,et al. Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column[J]. ELECTROPHORESIS,2008,29(8):1612-1618.
APA Jiang, Xiaogang.,Dong, Jing.,Wang, Fangjun.,Feng, Shun.,Ye, Mingliang.,...&邹汉法.(2008).Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column.ELECTROPHORESIS,29(8),1612-1618.
MLA Jiang, Xiaogang,et al."Automation of nanoflow liquid chromatography-tandem mass spectrometry for proteome and peptide profiling analysis by using a monolithic analytical capillary column".ELECTROPHORESIS 29.8(2008):1612-1618.
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