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A chemoenzymatic approach enables the site-specific conjugation of recombinant proteins 期刊论文
ELECTROPHORESIS, 2019, 卷号: 40, 期号: 16-17, 页码: 2125-2128
Authors:  Wang, Yan;  Dong, Mingming;  Ye, Mingliang
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Biorthogonal chemistry  Chemoenzymatic approach  Site-specific conjugation  Tyrosine phosphorylation  
Characterization of the substrate scope of an alcohol dehydrogenase commonly used as methanol dehydrogenase 期刊论文
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2019, 卷号: 29, 期号: 12, 页码: 1446-1449
Authors:  Guo, Xiaojia;  Feng, Yanbin;  Wang, Xueying;  Liu, Yuxue;  Liu, Wujun;  Li, Qing;  Wang, Junting;  Xue, Song;  Zhao, Zongbao K.
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Alcohol dehydrogenase  Substrate preference  Methanol dehydrogenase  Geobacillus stearothermophilus  Molecular docking  Cofactor regeneration  
Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein 期刊论文
JOURNAL OF SEPARATION SCIENCE, 2019, 卷号: 42, 期号: 3, 页码: 744-753
Authors:  Chang, Mengmeng;  Qin, Qian;  Wang, Bohong;  Xia, Tian;  Lv, Wangjie;  Sun, Xiaoshan;  Shi, Xianzhe;  Xu, Guowang
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immobilized metal-ion affinity chromatography  layer-by-layer assembly  magnetic nanoparticles  protein purification  
Tuning of acyl-ACP thioesterase activity directed for tailored fatty acid synthesis 期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 卷号: 102, 期号: 7, 页码: 3173-3182
Authors:  Feng, Yanbin;  Zhang, Yunxiu;  Wang, Yayue;  Liu, Jiao;  Liu, Yinghui;  Cao, Xupeng;  Xue, Song
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Acyl-acp Thioesterase  Medium-chain Fatty Acids  Fatty Acid Tailoring  Substrate Selectivity  Competitive Relationship  
Structural Insights into the Thermophilic Adaption Mechanism of Endo-1,4-beta-Xylanase from Caldicellulosiruptor owensensis 期刊论文
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2018, 卷号: 66, 期号: 1, 页码: 187-193
Authors:  Liu, Xin;  Liu, Tengfei;  Zhang, Yuebin;  Xin, Fengjiao;  Mi, Shuofu;  Wen, Boting;  Gu, Tianyi;  Shi, Xinyuan;  Wang, Fengzhong;  Sun, Lichao
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Gh10 Xylanase  Caldicellulosiruptor Owensensis  Thermostability  Crystal Structure  Loop 7  
Surface sieving coordinated IMAC material for purification of His-tagged proteins 期刊论文
ANALYTICA CHIMICA ACTA, 2017, 卷号: 997, 页码: 9-15
Authors:  Li, Senwu;  Yang, Kaiguang;  Liu, Lukuan;  Zhao, Baofeng;  Chen, Yuanbo;  Li, Xiao;  Zhang, Lihua;  Zhang, Yukui
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Surface Sieving  Metal Ion Affinity  Protein Purification  His-tag  
Surface sieving coordinated IMAC material for purification of His-tagged proteins 期刊论文
ANALYTICA CHIMICA ACTA, 2017, 卷号: 997, 页码: 9-15
Authors:  Li, Senwu;  Yang, Kaiguang;  Liu, Lukuan;  Zhao, Baofeng;  Chen, Yuanbo;  Li, Xiao;  Zhang, Lihua;  Zhang, Yukui
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Surface Sieving  Metal Ion Affinity  Protein Purification  His-tag  
Mixed-mode reversed phase/positively charged repulsion chromatography for intact protein separation 期刊论文
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2017, 卷号: 138, 页码: 63-69
Authors:  Ding, Ling;  Guo, Zhimou;  Hu, Zhuo;  Liang, Xinmiao
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Mixed-mode Stationary Phase  Reversed-phase Liquid Chromaitography  Positively Charged Repulsion  Intact Protein Separation  Click Chemistry  
Identification and characterization of naturally occurring inhibitors against UDP-glucuronosyltransferase 1A1 in Fructus Psoraleae (Bu-gu-zhi) 期刊论文
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2015, 卷号: 289, 期号: 4, 页码: 70
Authors:  Wang XX(王欣欣);  Lv X(吕侠);  Li SY(李世阳);  Hou J(侯洁);  Ning J(宁静);  Wang JY(王家悦);  Cao YF(曹云峰);  Ge GB(葛广波);  Guo B(郭斌);  Yang L(杨凌)
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Stirring rate regulates the proliferation and metabolism of microencapsulated recombinant CHO cells 期刊论文
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2015, 卷号: 62, 期号: 6, 页码: 833-839
Authors:  Wang, Yu;  Zhang, Ying;  Li, Na;  Chen, Li;  Zhang, Demeng;  Sun, Dongsheng;  Li, Shen;  Guo, Xin;  Ma, Xiaojun
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Cell Culture  Microencapsulated  Recombinant Protein Expression  Stirring Rate